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ATCC
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ATCC
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ATCC
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ATCC
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ATCC
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ATCC
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ATCC
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Journal: Translational Cancer Research
Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells
doi: 10.21037/tcr-2025-aw-2290
Figure Lengend Snippet: The role of miR-27a-3p in osteosarcoma cells. (A) Expression levels of miR-27a-3p in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2 and MG-63. (B,C) The influence of introducing miR-27a-3p mimic and inhibitor on the expression of miR-27a-3p in osteosarcoma cell lines MG-63 and SAOS-2. (D-F) CCK-8 assay, Transwell migration assay and FCM assay were used to detect the changes in cell proliferation, migration ability and apoptosis levels of osteosarcoma cells MG-63 and Saos-2 transfected with miR-27a-3p mimic and inhibitor. Stained with crystal violet; magnification: ×200. *, P<0.05; **, P<0.01. CCK-8, Cell Counting Kit-8; FCM, flow cytometry; NC, negative control.
Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal
Techniques: Expressing, CCK-8 Assay, Transwell Migration Assay, Migration, Transfection, Staining, Cell Counting, Flow Cytometry, Negative Control
Journal: Translational Cancer Research
Article Title: Molecular mechanism of miR-27a-3p targeting FBXW7 regulating the malignant behavior of osteosarcoma cells
doi: 10.21037/tcr-2025-aw-2290
Figure Lengend Snippet: The expression of FBXW7 in osteosarcoma cells. (A) The mRNA levels of FBXW7 in normal osteoblasts hFOB1.19 and osteosarcoma cells Saos-2, MG-63 were also analyzed. (B,C) To explore the impact of FBXW7 on the biological behavior of osteosarcoma cells Saos-2 and MG-63, the researchers performed transfections on these two osteosarcoma cell lines using pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7. (D,E) Effect of transfection of pcDNA3.1-FLAG-FBXW7 and siRNA-FBXW7 on expression of FBXW7 and β-catenin in osteosarcoma cells MG-63 (D) and Saos-2 (E). *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; ns, not significant. NC, negative control; OE, overexpression.
Article Snippet: Osteosarcoma cell lines MG-63 (RRID: CVCL_0426) and Saos-2 (RRID: CVCL_0548), as well as the normal
Techniques: Expressing, Transfection, Negative Control, Over Expression
Journal: Dentistry Journal
Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings
doi: 10.3390/dj14040202
Figure Lengend Snippet: Viability of human fetal osteoblasts (hFOB cell line) after 8 days of incubation on the surface of uncoated and coated TiZr samples. The results are calculated as mean ± standard deviation from three independent experiments and are presented relative to the control—the plastic surface of the plate with 96 wells; * p < 0.05 and ** p < 0.01 compared to control.
Article Snippet:
Techniques: Incubation, Standard Deviation, Control
Journal: Dentistry Journal
Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings
doi: 10.3390/dj14040202
Figure Lengend Snippet: Fluorescent staining of live cells (green labeling with calceine solution) and dead cells (red labeling with propidium iodide) after exposure of human osteoblasts to TiZr surfaces coated with Ag and HAP. All images were obtained with the 20× objective, scale bar: 50 μm.
Article Snippet:
Techniques: Staining, Labeling
Journal: Dentistry Journal
Article Title: Study on the Influence of Hydroxyapatite on Human Cell Viability and Adhesion in Chemical Antibacterial Silver Coatings
doi: 10.3390/dj14040202
Figure Lengend Snippet: Fluorescent labeling of actin filaments in human osteoblasts after 8 days of incubation with Ag- and HAP-coated samples (green: F-actin labeled with phalloidine-fluorescein isothiocyanate; blue: nuclei labeled with 4′,6-diamidino-2-phenylindole; scale bar: 100 μm).
Article Snippet:
Techniques: Labeling, Incubation